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Question

Mention 5 steps necessary in the collection of specimens in bacteriology lab.​

Answer

We need to follow specific steps to prepare specimens for examination under the microscope, including selecting specimens, treating with prefixation, fixing with chemicals, dehydrating, and drying with either critical-point drying or freeze-drying. Explanation: In order to properly observe specimens under a microscope, the specimens must be prepared in a precise manner. Firstly, we carefully select the specimens that will be studied. Then, we treat them with a prefixation solution, which allows the specimens to better retain their shape during the later stages of preparation. After that, we fix the specimens with chemicals to prevent any further degradation or changes in their structure. Next, we must dehydrate the specimens to remove any excess water. This is often done by placing the specimens in a series of increasing concentrations of alcohol or other dehydrating agents. Finally, we have two options for drying the specimens: critical-point drying or freeze-drying. Critical-point drying involves using a high-pressure chamber to dry the specimens, while freeze-drying involves freezing the specimens and then removing the excess water through sublimation. By following these precise steps, we can ensure that the specimens are prepared properly and can be accurately observed under the microscope.