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. which restriction enzyme did you choose? why did you choose that one? 2. where would you insert the insulin gene, and why? 3. which antibiotic would you use to determine if the recombinant dna was taken in?


In DNA cloning, the use of restriction enzymes and DNA ligase is necessary to splice genes and other DNA fragments into plasmids. Type II nucleases, well-known in molecular biology, are regularly employed in DNA fragmentation, analysis, and gene cloning. These enzymes break DNA at specific sites based on their recognition sequence, resulting in reproducible fragments and unique gel electrophoresis patterns. The most practical type II restriction endonucleases for laboratory use are those that cleave within their recognition site. Type I enzymes break DNA randomly, while type II endonucleases cut at predetermined sites, making them indispensable tools in genetic engineering. Although type I Renewables are necessary for bacterial operation, they do not cleave DNA at specific sites. On the other hand, type II restriction endonucleases, described in this document, require precise sites for DNA cleavage. To learn more about genes, take a look at brainly.com/question/8832859 #SPJ4.